Table 2.
Cytotoxicity Results of Pyrroloiminoquinone Alkaloids in the CCK-8 Cytotoxicity Assay under Hypoxic Conditions in Three Cancer Cell Lines
| ICSO value (μM) ± SEMa | ||||
|---|---|---|---|---|
| compound | HCT 116 (18 h, 0.5% O2) | HCT 116 (48 h, 0.5% O2) | LNCaP (18 h, 1% O2) | COLO 205 (48 h, 0.5% O2) |
| tirapazamine (positive control) | 72.7 ± 6.9 | 11.9 ± 1.3 | -c | 47.7 ± 12.2d |
| 1 | 3.1 ± 1.4 | NA | 7.0 ± 1.3 | 1.8 ± 1.5 |
| 2 | >10 | 26.8 ± 5.4b | >10 | >10 |
| 3 | 8.6 ± 0.3b | NA | >10 | 5.1 ± 1.1 |
| 5 | 7.2 ± 1.9 | NA | >10 | >10 |
| 8 | >10 | >50 | >10 | >10 |
| 9 | 6.2 ± 2.2 | >50 | 8.3 ± 2.5 | 7.6 ± 1.2 |
| 11 | >10 | NA | >10 | >10 |
a
IC50 values are presented as the average of triplicate experiments ± SEM (n = 3 or 4).
b
Outlier excluded (duplicate experiment).
c
IC50 not calculated: only one concentration tested (25 μM).
d
IC50 based on quintuplicate experiments (n = 3 or 4).