KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Anti-V5 antibody. Polyclonal | Bethyl labs | Catalog #A190–220A |
| Anti-Myc antibody, Monoclonal | EMD Millipore | Catalog # 050419 |
| Anti RNAPII Ser2 monoclonal, (Clone 9E10) | Active Motif | Catalog # 61084 |
| Anti-Spt6, polyclonal | In House | |
| Anti-histone H3 antibody, polyclonal | In House | |
| Anti-histone H2B antibody, polyclonal | Active Motif | Catalog # 39328 |
| Normal rabbit serum | Cell Signaling Technology | Catalog # 2729S |
| Anti-G6PDH antibody, polyclonal | Sigma | Catalog # A9521–1VL |
| ChIP grade RNAPII antibody | BioLegend | Catalog # 664912 |
| HRP-conjugated anti-rabbit | GE Healthcare | Catalog # NA934V |
| HRP-conjugated anti-mouse | GE Healthcare | Catalog # NA931V |
| Chemicals, Peptides, and Recombinant Proteins | ||
| 6-Azauracil | SIGMA (Roche) | Catalog # A1757 |
| Benomyl | SIGMA (Roche) | Catalog # 45339 |
| Hydroxyurea | SIGMA (Roche) | Catalog # H8627 |
| RNAseH | Promega | Catalog # M4281 |
| T4 Polynucleotide Kinase | NEB | Catalog # M0201S |
| ULTRAhyb Oligo Buffer | Thermo Fisher Scientific | Catalog # AM8663 |
| SSIII RT reverse transcriptase | Thermo Fisher Scientific | Catalog # 18080–044 |
| Acid phenol Chloroform | Ambion/Thermo Fisher Scientific | Catalog # AM9722 |
| Zeta Probe Blotting membrane | Biorad | Catalog # 1620153 |
| Rapamycin | SIGMA (Roche) | Catalog # R8781 |
| cOMPLETE, EDTA free protease inhibitor tablets | SIGMA (Roche) | Catalog # 11873580001 |
| PhosSTOP phosphatase inhibitor tablets | SIGMA (Roche) | Catalog # 04906845001 |
| Century TM Plus RNA markers | Thermo Fisher Scientific | Catalog # AM7145 |
| Gel Loading Buffer II | Thermo Fisher Scientific | Catalog # AM8547 |
| iTaq™ Universal SYBR ® Green Supermix | Biorad | Catalog # 172–5124 |
| Protein A Agarose | SIGMA (Roche) | Catalog # 11134515001 |
| Propidium Iodide | Thermo Fisher Scientific | Catalog # P3566 |
| Thiolutin | Abcam | Catalog # ab143556 |
| Dynabeads Protein G | Thermo Fisher Scientific | Catalog # 10009D |
| SPRI Beads (Agencourt AMPure XP) | Beckman Coulter | Catalog # A63881 |
| α-Factor Mating pheromone | Genscript | Catalog # 59804–28–4 |
| Critical Commercial Assays | ||
| TruSeq Stranded Total RNA with RiboZero Gold Library Prep Kit. | Illumina | Catalog # RS-122–2301 |
| Deposited Data | ||
| RNAseq and ATAC seq data | This study | GEO:GSE111815 |
| Raw data for Northern Blots and Immunoblots | This Study | Mendeley:http://dx.doi.org/10.17632/tnx4py69j8 |
| Experimental Models: S.serevisiae strains | ||
| MATa leu2 trp1-Δ63 ura3 his4–912∂ lys2–128∂ | Winston Lab | FY2181 |
| MATa leu2 trp1-Δ63 ura3 his4–912∂ lys2–128∂ spt6–1004 | Winston Lab | FY2180 |
| MATa leu2 trp1-Δ63 ura3 his4–912∂ lys2–128∂ CCR4–9MYC::kanMX | This study | RDY20171 |
| MATa leu2 trp1-Δ63 ura3 his4–912∂ lys2–128∂ spt6tSH2::natMX | This study | RDY20172 |
| MATa leu2 trp1-Δ63 ura3 his4–912∂ lys2–128∂ spt6tSH2::natMX CCR4–9MYC::kanMX | This study | RDY20173 |
| MATa ade2–1 trp1–1can1–100 leu2–3, 112 his3–11, 15 ura3 tor1–1 frp1::NAT RPL13A-2XFKBP12::TRP1 SPT6-FRB::KanMX | This study | yDZ001 |
| Oligonucleotides | ||
| Oligos | This study and other sources | Supplementary Table 2 |
| Recombinant DNA | ||
| NOT1-V5 (plasmid), | DNASU | Catalog # ScCD00102519 |
| NOT2-V5 (plasmid) | DNASU | Catalog # ScCD00103149 |
| DHH1-V5 (plasmid) | DNASU | Catalog # ScCD00102795 |