Fig. 5.

miR-103a-3p/miR-382-5p regulated the viability, migration and tube formation of GECs and Hsp90β expression by targeting ZIC4 3’-UTR. a-d The mRNA and protein expressions of ZIC4 were regulated by miR-103a-3p/miR-382-5p. Values represent the means ± SD (n = 5, each group). **P < 0.01 vs. miR-103a-3p/miR-382-5p (+) NC group. ^##P < 0.01 vs. miR-103a-3p/miR-382-5 (−) NC group. e-f The putative binding sites between ZIC4 and miR-103a-3p/miR-382-5p were predicted respectively and the relative luciferase activity was expressed as firefly/renilla luciferase activity. Values are means ± SD (n = 5, each group). **P < 0.01 vs. ZIC4 Wt + miR-103a-3p/miR-382-5p (+) NC group. g-h The co-effects of miR-103a-3p/miR-382-5p and ZIC4 on the viability of GECs was evaluated by CCK8 assay. Data are presented as the means ± SD (n = 5, each group), **P < 0.01 vs. miR-103-3p / miR-382-5p + ZIC4 group; ^##P < 0.01 vs. miR-103-3p/miR-382-5p + ZIC4-NC group. i-j The co-effects of miR-103a-3p/miR-382-5p and ZIC4 on the migration of GECs were evaluated by Transwell assay. Data are presented as the means ± SD (n = 5, each group), **P < 0.01 vs. miR-103-3p / miR-382-5p + ZIC4 group; ^##P < 0.01 vs. miR-103-3p/miR-382-5p + ZIC4-NC group. k-l The co-effects of miR-103a-3p/miR-382-5p and ZIC4 on the tube formation of GECs were evaluated by Matrigel tube formation assay (black arrows, tube structures; gray arrows, tube branches). Data are presented as the means ± SD (n = 5, each group), **P < 0.01 vs. miR-103-3p / miR-382-5p + ZIC4 group; ^##P < 0.01 vs. miR-103-3p/miR-382-5p + ZIC4-NC group. m-n The co-effect of miR-103a-3p/miR-382-5p and ZIC4 on expression of Hsp90β by western blot assay. Data are presented as the means ± SD (n = 3, each group), **P < 0.01 vs. miR-103-3p / miR-382-5p + ZIC4 group; ^##P < 0.01 vs. miR-103-3p/miR-382-5p + ZIC4-NC group