Table 2.
Primers and probes used for RT-qPCR
| Primer or Probe | Gene | Sequence (5′- > 3′) or Assay ID |
|---|---|---|
| Primer | circ-DICER1 | F: TGTAATAATTGTAGCCAAGTAAATCTCC |
| R: AAGTAACCATTTTTCAAAACATTCAAG | ||
| P:FAM + TAAAGTTATCGTCTGTTCAGTTCTCATTATGACTTG+BHQ1 | ||
| GAPDH | F:GGACCTGACCTGCCGTCTAG | |
| R:TAGCCCAGGATGCCCTTGAG | ||
| P:FAM + CCTCCGACGCCTGCTTCACCACCT+Eclipse | ||
| MOV10 | F: CCATGAGGCACATTGTTACG | |
| R:AAGTGCTTCACCACCTGCTT | ||
| ZIC4 | F:CTAGCGACAAGCCATACACG | |
| R:GTAGCCGAATCGTAGCCAGA | ||
| Hsp90β | F:GTGGGTTCAGATGAGGAGGA | |
| R:TCTGGTCCAAATAGGCTTGG | ||
| Probe | MiR-103a-3p | 000439(Applied biosystems) |
| MiR-382-5p | 000572(Applied biosystems) | |
| U6 | 001973(Applied biosystems) |