Fig. 6.

Effect of diet and topiramate on tight junction protein expression using immunofluorescence. Immunohistochemical techniques were used on tissue from the hypothalamus to examine ZO-1 (a) and occludin (b) expression in LF- and HF-fed CD-1 mice treated with or without TPM (a). ZO-1 and occludin expression were significantly decreased with HF-diet feeding compared to LF-fed controls. TPM treatment attenuated ZO-1 expression. GLUT1 was used as a vessel marker for ZO-1 and CAV1 as a vessel marker for occludin. The merged image localizes the tight junction staining to the vessels. Scale bar is 50 microns. Values are expressed as mean ± SD. Significance was determined by a one-way analysis of variance followed by Newman–Keuls post-test between HF vs. LF, LF vs. LF TPM, and HF vs. HF TPM. *p < 0.05