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. 2019 Jan 7;50:2. doi: 10.1186/s13567-018-0619-6

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© The Author(s) 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Electropherogram (PAGE) of selected passages in MA104 cells of three porcine RVA strains. The RNA genomic segments of three vaccine strains from different passage numbers were separated by PAGE and visualized using silver staining. The original virulent strains and each passage of 174-1 (A), K71 (B), and PRG942 (C) demonstrated typical RVA’s RNA segment patterns of 4-2-3-2 and maintained their own patterns throughout the serial passages. Lane 1, original virulent strain; lane 2, 10^th passage; lane 3, 20^th passage; lane 4, 40^th passage; lane 5, 60^th passage; lane 6, 80^th passage.