Table 1.
Primers for Real-Time PCR
| Gene Name | Gene Symbol | Forward Primer | Reverse Primer |
|---|---|---|---|
| Mouse Pomc | Pomc | TGTACCCCAACGTTGCTGAG | AGGACCTGCTCCAAGCCTAA |
| Mouse Crhr1 | Crhr1 | GGGCAGCCCGTGTGAATTAT | GAAGAGGACAAAGGCCACCA |
| Mouse T-box 19 | TBX19, Tpit | CCTTTCGCCAAGGCCTTCTT | AGTAGGTCACATGCTGGCTC |
| Mouse paired-like homeodomain transcription factor 1 | Pitx1 | GAGAACTCCGCCAGCGAATC | CTCTGGCCCCTTGGCTTC |
| Mouse neuronal differentiation 1 | Neurod1 | GGCTCCAGGGTTATGAGATCG | CGCTCTCGCTGTATGATTTGG |
| Mouse growth hormone | Gh | GCTTCTCAGAGACCATCCCG | ATCCTGCTGAGGAACTGCAC |
| Mouse prolactin | Prl | AGAGCTGTTTGACCGTGTGG | GTAGCCAGGGAAGAAGTGGG |
| Mouse thyroid-stimulating hormone β subunit | Tshβ | GAGAGTGTGCCTACTGCCTG | ACAGACATCCTGAGAGAGTGC |
| Mouse luteinizing hormone β subunit | Lhβ | TCTGCATCACCTTCACCACC | CTGAGGCACAGGAGGCAAA |
| Mouse follicle-stimulating hormone β subunit | Fshβ | ATACCACTTGGTGTGCGGG | TACTTTCTGGGTATTGGGCCG |
| Mouse 18S ribosomal RNA | 18s RNA | TGGCCAACGGTCTAGACAAC | CAGTGGTCTTGGTGTGCTGA |
| Human POMC | POMC | CAGGACCTCACCACGGAAAG | CGGGAACATGGGAGTCTCG |
| Human CRHR1 | CRHR1 | CAACTACCTGGGCCACTGTATC | CATCTGCCTGGTCACCCCAAT |
| Human 18S ribosomal RNA | 18s RNA | GTTCAGCCACCCGAGATTGA | CTGAGCCAGTCAGTGTAGCG |