TABLE 1.
Antibodies Used for Immunohistochemical Analysis
| Antibody | Type of Antigen | Manufacturer | Dilution |
|---|---|---|---|
| CD2 | Pan-T cell | Novocastra Laboratories, Newcastle Upon Tyne, UK | 1:20 |
| CD3 | Pan-T cell | Ventana Medical Systems, Tucson, AZ | Prediluted |
| CD4 | T helper | Novocastra Laboratories | 1:20 |
| CD5 | Pan-T cell | Ventana Medical Systems | Prediluted |
| CD7 | Pan-T cell | Dako, Carpinteria, CA | Prediluted |
| CD8 | Cytotoxic | Ventana Medical Systems | Prediluted |
| CD10 | Germinal center | Ventana Medical Systems | Prediluted |
| CD15 | CHL | Ventana Medical Systems | Prediluted |
| CD20 (L26) | B cell | Ventana Medical Systems | Prediluted |
| CD25 | T-regulatory | Novocastra Laboratories | 1:150 |
| CD30 | Classical Hodgkin lymphoma | Ventana Medical Systems | Prediluted |
| CD43 | Pan-T cell | BioGenex, San Ramon, CA | 1:40 |
| CD45RA | Naïve T cell | Dako | 1:30 |
| CD45RO | Pan-T cell, memory T cell | Ventana Medical Systems | Prediluted |
| CD57 | Follicular T-helper | Ventana Medical Systems | Prediluted |
| Ki67 | Proliferation | Ventana Medical Systems | Prediluted |
| bcl-6 | Germinal center | Dako | 1:10 |
| IgD | Immunoglobulin heavy chain subclass | Cell Marque, Rocklin, CA | Prediluted |
| Granzyme B | Cytotoxic | Chemicon International, Chemicon, CA | 1:10 |
| Perforin | Cytotoxic | Lab Vision Products, Freemont, CA | 1:20 |
| FOXP3 | T-regulatory | Abcam, Cambridge, MA | 1:800 |
| T-bet | T-helper 1 | Santa Cruz Biotechnology, Santa Cruz, CA | 1:100 |
| PD-1 | Follicular T-helper | Cell Marque | 1:50 |