Table 1.
Rough epitope mapping of AG3.0
| SIVmac251 Gag Capsid Sequence | |||||
| 001 | PVQQIGGNYV | HLPLSPRTLN | AWVKLIEEKK | FGAEVVPGFQ | ALSEGCTPYD |
| 051 | INQMLNCVGD | HQAAMQIIRD | IINEEAADWD | LQHPQPAPQQ | GQLREPSGSD |
| 101 | IAGTTSSVDE | QIQWMYRQQN | PIPVGNIYRR | WIQLGLQKCV | RMYNPTNILD |
| 151 | VKQGPKEPFQ | SYVDRFYKSL | RAEQTDAAVK | NWMTQTLLIQ | NANPDCKLVL |
| 201 | KLGLVNPTLE | EMLTACQGVG | GPGQKARL | ||
| Peptidea | Sequence | Mean OD | StDev |
|---|---|---|---|
| 1 | 01–20 | 0.01 | 0.00 |
| 2 | 11–30 | 0.37 | 0.05 |
| 3 | 21–40 | 0.01 | 0.00 |
| 4 | 31–50 | 0.01 | 0.00 |
| 5 | 41–60 | 0.01 | 0.00 |
| 6 | 51–70 | 0.01 | 0.00 |
| 7 | 61–80 | 0.01 | 0.00 |
| 8 | 71–90 | 0.01 | 0.00 |
| 9 | 81–100 | 0.01 | 0.00 |
| 10 | 91–110 | 0.01 | 0.00 |
| 11 | 101–120 | 0.01 | 0.00 |
| 12 | 111–130 | 0.01 | 0.00 |
| 13 | 121–140 | 0.01 | 0.00 |
| 14 | 131–150 | 0.01 | 0.00 |
| 15 | 141–160 | 0.01 | 0.00 |
| 16 | 151–170 | 0.01 | 0.00 |
| 17 | 161–180 | 0.01 | 0.00 |
| 18 | 171–190 | 0.01 | 0.00 |
| 19 | 181–200 | 0.01 | 0.00 |
| 20 | 191–210 | 0.01 | 0.00 |
| 21 | 201–220 | 0.01 | 0.00 |
| 22 | 211–228 | 0.01 | 0.00 |
a
Peptides were ordered from the European AIDS Reagent Programme, reference ARP714.1–22; peptide 15, 16, and 18 have an additional N-terminal cysteine)