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Knockdown of ESRP1 induces EMT in H1975 cells. (a) Immunofluorescence images for hybrid E/M H1975 cells—mock (leftmost column), treated with control siRNA (2nd column from the left), and treated with two independent siRNAs targeting ESRP1 (3rd and 4th columns from the left). (b) Western blot showing changes in ESRP1, CDH1, VIM, and ZEB1 levels. (c) Bright-field microscopy images of mock (top) and si-ESRP1 #2 treated H1975 cells (bottom).
