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Schematic of the mechanism of metastasis and the experimental design. (a) Stromal cells and cancer cells form hybrids spontaneously in vivo; such hybrids contribute to metastatic mass. The hybrids are well-equipped to migrate out of the primary tumor and reestablish themselves at secondary sites. (b) Scheme to evaluate the metastatic potential of tumor-stroma hybrids in vitro. (c) Scheme to evaluate the metastatic potential of tumor-stroma cell hybrids in vivo.
