Figure 1.

PYR/PYL/RCAR-Like Genes in Marchantia polymorpha. A, Phylogenetic analysis of PYR/PYL/RCAR-related proteins in Arabidopsis, Amborella trichopoda, Picea abies, Selaginella moellendorffii, Physcomitrella patens, and M. polymorpha. Amino acid sequences were aligned with MUSCLE. Phylogenetic relationship among PYR/PYL/RCAR-like proteins was inferred using the neighbor-joining method. Numbers on the branches indicate bootstrap values (100 replicates). The bar represents the number of amino acid changes per branch length. Phylogenetic analyses were conducted in MEGA7. B, Effect of overexpression of MpPYL1 on ABA-induced gene expression. The cDNA of MpPYL1 fused with the rice actin promoter was introduced into the P. patens protonemata cells by particle bombardment. The ABA-inducible Em promoter fused with the GUS reporter gene was used as a reporter construct. The tissues were incubated with or without ABA (10 µM) and used for fluorometric GUS assays. GUS activity was normalized by expression of the cointroduced luciferase (LUC) reporter gene. Results of PYL6 of Arabidopsis (AtPYL6, At2g40330) are shown for comparison. *P < 0.05 and ***P < 0.001 by the t test.