Figure 5.

OHP1, OHP2, and HCF244 exhibit a leaf age-dependent reduction and are induced by high light. A, Immunodetection of the OHP1, OHP2, and HCF244 proteins during leaf development. Five-week-old wild-type plants were used in this study. The leaves corresponding to lanes 1 to 5, representing the leaves from old to young, are shown in Supplemental Figure S4. Thylakoid membranes were loaded on an equal protein level and probed with the indicated antibodies. B, Immunodetection of OHP1, OHP2, and HCF244 under stress conditions. HL, HT, and LT represent high light, high temperature, and low temperature, respectively. After treatment, thylakoids were isolated and subjected to SDS-urea-PAGE analysis. The proteins were probed with the indicated antibodies. An immunoblot with an antibody against CF₁α/β was used as a loading control.