Figure 4.

Fe accumulation patterns in roots of wild-type seedlings at 0 to 5 DAT from +Pi medium to +Pi or −Pi medium. Fe accumulation was assessed by Perls/DAB staining. Wild-type seeds were first germinated on +Pi medium for 3 d and then transferred to +Pi medium (A) or −Pi medium (B). The top, middle, and bottom rows in each section are photographs of the whole root (the red lines indicate the positions of the EZ), a part of the MZ, and the root tip, respectively. The root images of the top rows are composed of two tiled images. Roots of representative seedlings are shown. Bars = 100 μm.