Figure 5.

Fe accumulation patterns in the roots of the wild type (WT), various mutants and overexpressing lines, and the stop1 als3 and almt1 als3 double mutants as indicated by Perls/DAB staining. Seeds of the different genotypes were germinated on +Pi medium and grown for 3 d before they were transferred to +Pi medium (A) or −Pi medium (B). At 2 DAT, the roots of the seedlings were stained for Fe. The top, middle, and bottom rows in each section are photographs of the whole root, a part of the MZ, and the root tip, respectively. The root images of the top rows are composed of two tiled images. Roots of representative seedlings are shown. Bars = 100 μm.