Figure 7.

Transcriptional regulation of STOP1 and ALMT1 by ALS3. A and B, RT-qPCR analyses of the expression of STOP1 (A) and ALMT1 (B) in the roots of 6-d-old seedlings of the wild type (WT) and als3. C and D, Expression patterns of STOP1::GUS (C) and ALMT1::GUS (D) transgenes in the roots of 6-d-old seedlings in the wild-type and als3 backgrounds. The top row in each section shows whole seedlings, and the bottom row shows root tips. Bars = 1 cm (top rows) and 100 μm (bottom rows). E and F, RT-qPCR analyses of the expression of STOP1 (E) and ALMT1 (F) in 6-d-old wild type and 35S::ALS3-STAR1 seedlings. In A, B, E, and F, ACTIN2 was used as an internal control. The experiments were repeated three times, and representative results are shown. The values are means + sd of three samples each with three technical replicates. Asterisks indicate significant differences from the wild type (Student’s t test, ****, P < 0.0001).