Skip to main content
. 2018 Dec 29;4:e00081. doi: 10.1016/j.parepi.2018.e00081

Fig. 2.

Fig. 2

Agarose electrophoresis of Acanthamoeba-specific-PCR.

(A) PCR of extracted DNA with protocol I–V.

Lane 1) 100 bp ladder (Nippon gene, Japan), Lane 2) Protocol I, Lane 3) Protocol II, Lane 4) Protocol III, Lane 5) Protocol IV, Lane 6) Protocol V.

(B) PCR of spike sample DNA extracted with protocol V.

Lane 1) 100 bp ladder (Nippon gene, Japan), Lane 2) 1.0 × 105 amebas/10 g soil, Lane 3) 1.0 × 104 amebas/10 g soil, Lane 4) 1.0 × 103 amebas/10 g soil, Lane 5) 1.0 × 102 amebas/10 g soil, Lane 6) 1.0 × 101 amebas/10 g soil.

(C) PCR of soil No. 1-7 DNA extracted with protocol V.

Lane 1) 100 bp ladder (Nippon gene, Japan), Lane 2) No. 1, Lane 3) No. 2, Lane 4) 1 No. 3, Lane 5) No. 4, Lane 6) No. 5, Lane 7) No. 6, Lane 8) No. 7.