Table 2.
Summary of significant gene expression changes in the 11B7501 B lymphoma cell line. Up- or downregulation is described as the percentage of expression relative to the negative controls of ß2M. The ß2M negative control (-EE2/-LPS) was used to normalize genes exposed to EE2 only, whereas the ß2M negative control (-EE2/+LPS) was used to normalize genes exposed to EE2 and LPS.
| Genes | 25 000 μg/L EE2 | 25 000 μg/L EE2 + 5 μg/mL LPS |
|---|---|---|
| AHR | ⬇ 81±12 % | ⬆ 120±13 % |
| CD9 | ⬇ 59±7 % | |
| HSP70 | ⬆ 191±36 % | ⬆ 228±80 % |
| IFNγ | ⬆ 141±13 % | |
| LYN1 | ⬇ 63±14 % | ⬆ 142±17 % |
| MAPKK3 | ⬆ 163±48 % | |
| SLAM | ⬇ 63±29 % | |
| TGFß1 | ⬇ 62±15 % |
EE2=17α-ethinyl estradiol; LPS=lipopolysaccharide; AHR=aryl hydrocarbon receptor; CD9=Cluster of differentiation 9; HSP70=70 kDa heat shock protein; IFNγ=interferon gamma; LYN1=Lck/Yes novel tyrosine kinase; MAPKK3=mitogen-activated protein kinase kinase; SLAM=signaling lymphocytic activation molecule; TGFß1=transforming growth factor beta 1.