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. Author manuscript; available in PMC: 2019 Jul 1.
Published in final edited form as: Pept Sci (Hoboken). 2018 Aug 21;110(4):e24074. doi: 10.1002/pep2.24074

Figure 6. Membrane permeabilzation and cytoxicity assays.

Figure 6

(A) Flow cytometry analysis of PI leakage into S. aureus after treatment with peptides. The percent permeabilization was determined from PI intensity. (B) Hemolysis of sheep RBCs by peptides PBP (red), PBP-W11 (black), PBP-W18 (green), PBP-X (purple), and PBP-A (blue). The percent permeabilization was determined by comparing the absorbance at 415nm in samples treated with peptides to that of samples treated with 8mM CTAB (set at 100% leakage). Error bars are included but are obscured by the symbols in all cases. Standard deviations were <1% in all cases. (C) Cytoxicity against HeLa cells measured by CTB assay. Cells were incubated with varying concentrations of peptide for 24 h and cell viability was assessed. Representative data from one of two biologicial replicates shown, all samples normalized to cytotoxicity of positive control CTAB.