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. 2019 Feb;95(2):155–168. doi: 10.1124/mol.118.113233

Fig. 1.

Fig. 1.

AM1710 inhibited forskolin-stimulated cAMP in HEK cells expressing mCB2 and hCB2, but the kinetics of inhibition differed between mCB2 and hCB2. (A) In HEK cells expressing mCB2, both CP55940 and AM1710 reduced cAMP levels at 5 minutes. The inhibitory effect of AM1710 lasted longer than CP55940 and dissipated by 15 minutes. (B) After treating HEK cells expressing mCB2 with PTX, both CP55940 and AM1710 failed to reduce cAMP levels at all time points examined. (C) In HEK cells expressing hCB2, CP55940 induced early reduction of cAMP at 5 minutes, which lasted up to 10 minutes, whereas AM1710 induced a delayed (at 10 minutes) but long-lasting (up to 30 minutes) decrease in cAMP. (D) After treating HEK cells expressing hCB2 with PTX, both CP55940 and AM1710 failed to reduce cyclase levels at all time points examined. *P < 0.05 vs. No Fsk; #P < 0.05 vs. Veh + Fsk, ^P < 0.05 significant difference between CP + Fsk and AM1710 + Fsk (two-way mixed ANOVA, followed by Bonferroni’ post hoc test). AU, arbitrary unit; CP, CP55940; Fsk, forskolin; hCB2, human CB2 receptors; mCB2, mouse CB2 receptors; Veh, vehicle. n = 3 for each group.