Fig. 1.

XBP1 splicing during incubation of primary human and mouse hepatocytes with EFV and 8-OHEFV. Representative gel images and sXBP1/uXBP1 densitometry quantification ratios of XBP1 mRNA splicing from semiquantitative reverse transcriptase PCR in (A) human primary hepatocytes (n = 6) and (B) male mouse primary hepatocytes (n = 6) in the presence of EFV or 8OH-EFV at 10 or 50 μM for 4 hours. Data are the mean ± S.D. Ratios of sXBP1/uXBP1 densitometry were log-transformed and the statistical significance of TM, EFV, or 8-OHEFV treatments from DMSO (0.1%) control was determined using an unpaired t test on the transformed data, generating two-tailed P values (*P < 0.05; ***P < 0.001).