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. 2019 Feb;95(2):183–195. doi: 10.1124/mol.118.113647

Fig. 7.

Fig. 7.

XBP1 splicing and lipid droplet formation in male mouse primary hepatocytes during EFV exposure. (A) Representative images (20×) of Oil Red O (red)- and hematoxylin (blue)-stained primary mouse hepatocytes and quantitation of Oil Red O signal intensity in WT (n = 4) primary mouse hepatocytes in presence of 20 μM EFV for 8 hours ± STF 083010 at 100 μM (mean ± S.D.). Fold change in Oil Red O intensity was calculated with respect to the mean of the DMSO (0.1%) control and fold changes were log-transformed. (B) Representative XBP1 mRNA splicing PAGE images and densitometry quantification (mean ± S.D.) of XBP1 mRNA splicing from semiquantitative reverse transcriptase PCR for sXBP1 and uXBP1 in male mouse primary hepatocytes (n = 4) treated with EFV and analogs of EFV at 50 μM for 8 hours ± STF 083010 at 100 μM. Ratios of sXBP1/uXBP1 and p-IRE1α/total IRE1α densitometry were log-transformed. The statistical significance in both (A and B) was determined for all treatments with respect to DMSO (0.2%) control using an unpaired t test on transformed data, generating two-tailed P values (*P < 0.05; **P < 0.01; ***P < 0.001). Additional un-paired t tests were performed to compare treatments indicated by brackets generating two-tailed P values (##P < 0.01; ###P < 0.001). n.s., not significant.