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Ac-Nle-SP-probe activates the NK1 receptor constructs. IP1 accumulation was assessed in both Dox-induced and uninduced Flp-In T-REx 293 cells harboring either the HA-NK1-eGFP (A) or the HA-NK1-6xHis (B) construct. The Ac-Nle-SP-probe was clearly active, although less potent that native Ac-Nle-SP. Data are means ± S.D. pooled from n = 3 independent experiments, each performed in triplicate.
