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. 2018 Dec 18;9(99):37305–37318. doi: 10.18632/oncotarget.26468

Figure 3. Effect of atuveciclib on apoptosis in TNBC cell lines.

Figure 3

(A) MDA-MB-231 (left panel) or MDA-MB-453 (right panel) cells were treated with vehicle control (VC) or atuveciclib at indicated concentrations for 24 hours. Equal amounts of lysates were analyzed by SDS-PAGE and immunoblotted for antibodies against PARP antibody (which detects both cleaved (cPARP) and full length PARP) and HSP90. (B) MDA-MB-231 (left panel) or MDA-MB-453 (right panel) cells were treated with atuveciclib for 4 days at indicated concentrations. Cells were stained with Annexin-V and propidium Iodide (PI) antibodies and then analyzed by flow cytometry. Annexin-V positive and Annexin-V+PI double positive cells were considered apoptotic. Data are presented as the fold change (FC) over VC-treated cells. Results represent the means ± SEM of nine (MDA-MB-231) or six (MDA-MB-453) independent experiments. *P < 0.05; **P < 0.01; ****P < 0.0001. N.S. stands for non-significant.