Table 2. Descriptive statistical analysis of H3K27M, H3K27me3, and H3K27Ac immunohistochemical (IHC) staining results.
| H3K27M (n = 69) | H3K27me3 (n = 61) | H3K27Ac (n = 33) | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| MT = 38 | WT = 31 | p-value | Present = 40 | Absent = 21 | p-value | High = 24 | Medium = 4 | Low = 5 | p-value | |
| Age | 0.008** | 0.62 | 0.626 | |||||||
| <5 years | 9 (23.68) | 8 (25.81) | 13 (32.50) | 5 (23.81) | 11 (45.83) | 1 (25.00) | 1 (20.00) | |||
| 5–10 years | 19 (50.00) | 4 (12.90) | 10 (25.00) | 7 (33.33) | 5 (20.83) | 1 (25.00) | 3 (60.00) | |||
| >10 years | 10 (26.32) | 19 (61.29) | 17 (42.50) | 9 (42.86) | 8 (33.33) | 2 (50.00) | 1 (20.00) | |||
| WHO-Grade | 0.594 | 0.02* | 0.782 | |||||||
| I | 11 (28.95) | 9 (29.03) | 18 (45.00) | 2 (9.52) | 7 (29.17) | 1 (25.00) | 1 (20.00) | |||
| II | 4 (10.53) | 6 (19.35) | 5 (12.50) | 3 (14.29) | 2 (8.33) | 1 (25.00) | 0 (0.00) | |||
| III | 7 (18.42) | 7 (22.58) | 8 (20.00) | 5 (23.81) | High Grade 0.016* | 4 (16.67) | 1 (25.00) | 2 (40.00) | ||
| IV | 16 (42.11) | 9 (29.03) | 9 (22.50) | 11 (52.38) | 11 (45.83) | 1 (25.00) | 2 (40.00) | |||
| Gender | 0.230 | 0.59 | 0.025* | |||||||
| Male | 16 (42.11) | 18 (58.06) | 17 (42.50) | 11 (52.38) | 8 (33.33) | 4 (100.00) | 3 (60.00) | |||
| Female | 22 (57.89) | 13 (41.94) | 23 (57.50) | 10 (47.62) | 16 (66.67) | 0 (0.00) | 2 (40.00) | |||
| Anatomical Location | 0.001*** | 1 | 1 | |||||||
| Midline | Midline 0.012* | Midline 0.036* | ||||||||
| Brainstem | 16 (42.11) | 2 (6.45) | 8 (20.00) | 9 (42.86) | 7 (29.17) | 1 (25.00) | 1 (20.00) | |||
| Thalamus | 15 (39.47) | 12 (38.71) | 17 (42.50) | 10 (47.62) | 16 (66.67) | 3 (75.00) | 4 (80.00) | |||
| Non-midline | ||||||||||
| Cerebellar Hemisphere | 3 (7.89) | 6 (19.35) | 6 (15.00) | 1 (4.76) | 0 (0.00) | 0 (0.00) | 0 (0.00) | |||
| Cerebral Hemisphere | 4 (10.53) | 11 (35.48) | 9 (22.50) | 1 (4.76) | 1 (4.17) | 0 (0.00) | 0 (0.00) | |||
| Progression | (n = 65) | 0.397 | (n = 57) | 0.51 | (n = 32) | 0.643 | ||||
| Yes | 28 (80.00) | 21 (70.00) | 28 (75.68) | 17 (85.00) | 21 (91.30) | 4 (100.00) | 4 (80.00) | |||
| No | 7 (20.00) | 9 (30.00) | 9 (24.32) | 3 (15.00) | 2 (8.07) | 0 (0.00) | 1 (20.00) | |||
| Death | (n = 63) | 0.044* | (n = 55) | <0.001*** | (n = 30) | 1 | ||||
| Yes | 20 (60.61) | 10 (33.33) | 12 (32.43) | 16 (88.89) | 14 (67.67) | 3 (75.00) | 3 (60.00) | |||
| No | 13 (39.39) | 20 (60.67) | 25 (67.57) | 2 (11.11) | 7 (33.33) | 1 (25.00) | 2 (40.00) | |||
| Recurrence | (n = 17) | 0.637 | (n = 14) | 1 | (n = 3) | n/a | ||||
| Yes | 3 (17.65) | 6 (35.29) | 6 (54.55) | 1 (33.33) | 2 (67.33) | No data | No data | |||
| No | 4 (23.53) | 4 (23.53) | 5 (45.45) | 2 (66.67) | 1 (33.33) | |||||
Patient clinical characteristics and outcomes relative to tumor Histone H3K27M, H3K27me3, H2K27Ac staining results. Between groups comparison was performed using Fischer's exact test for statistical significance. KEY: MT = H3K27M positive, WT = H3K27M negative. *= statistical significance: *p ≤ 0.05, **p ≤ 0.01, **p ≤ 0.001, Fischer's exact test. Average H3K27Ac staining score ranked low (0-33%), medium (33.1-66%), and high (66.1-100%). Numbers in parentheses indicate percentage of total cohort.