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. 2018 Dec 14;9(98):37097–37111. doi: 10.18632/oncotarget.26365

Figure 6. TAGLN2 protein expression is higher in U87 MG (IDH1/2 WT) parental cells compared to U87 MG cells overexpressing IDH1 R132H mutant protein due to hypomethylation of TAGLN2 promoter.

Figure 6

(A) TAGLN2 protein levels were compared in U87 MG IDH1/2 WT parental cells and a commercially available U87 MG isogenic cell line overexpressing IDH1 with a heterozygous R132H mutation by Western blot analysis. TAGLN2 protein was decreased in IDH1 mutant cells compared to IDH1/2 WT cells. (B) U87 MG IDH1 mutant cells were treated with increasing concentrations of 5-azacytidine (5-AZA) demethylating agent and TAGLN2 protein was evaluated by Western blot. 5-AZA resulted in increasing TAGLN2 protein levels expression in a dose-dependent manner.