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. Author manuscript; available in PMC: 2019 Dec 6.
Published in final edited form as: Cell Stem Cell. 2018 Nov 15;23(6):869–881.e8. doi: 10.1016/j.stem.2018.10.010

Figure 6: Time-course analysis of cells during organoid differentiation reveals lineage relationships.

Figure 6:

(A,B) Projecting cells across time-points to the tSNE. Cells were colored by the time point where they were collected (A) or gene expression of stage specific markers (B). (C) Validation of the stage specific marker by qPCR. **p<0.01 and ****p<0.001 versus day 7. (D) Annotation of cell clusters based on gene expression of cell type specific markers. (E,F) Ordering of scRNA-seq expression data according to the pseudotemporal position along the lineage revealed a continuum of gene expression changes from iPSCs to differentiated cell types.