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. 2019 Jan 8;17(1):e3000068. doi: 10.1371/journal.pbio.3000068

Fig 2. A single microinjection of dsRNA against EOF1 promotes the abnormal eggshell phenotypes in multiple gonotrophic cycles.

Fig 2

(A) Schematic diagram of experimental time course for dsRNA microinjection, blood feeding, and oviposition in the first three gonotrophic cycles. Representative eggs are shown from mosquitoes microinjected with dsRNA-Fluc (B) and dsRNA-EOF1 (C). An effect of RNAi-EOF1 or RNAi-Fluc control on fecundity was studied during the first three gonotrophic cycles (D). Each dot represents the number of eggs oviposited by an individual mosquito (N = 30). The mean ± SE are shown as horizontal lines, and statistical significance is represented by stars above each column (unpaired Student's t test; ***p < 0.001; **p < 0.01). An effect of RNAi-EOF1 on viability was also examined during the first three gonotrophic cycles (E). A significant reduction in the percentage of egg hatching was observed in RNAi-EOF1 mosquitoes. Each bar corresponds to egg viability from 15 individual mosquitoes from both groups. Underlying data can be found in S1 Data. dsRNA, double-stranded RNA; EOF1, eggshell organizing factor 1; Fluc, firefly luciferase; RNAi, RNA interference; SE, standard error.