Table 3. Properties of CHOS fractions derived from 1H-NMR and SEC.
| Fraction | C32 | C17 | C31 | C54 | C62 |
|---|---|---|---|---|---|
| cutoff (kDa)a | n.a. | < 3.5 | >3.5 <8 | >3.5 | >10 |
| 1H-NMR (DPn)b | 32 | 17 | 31 | 54 | 62 |
| MW (kDa)c | 15 | 7.6 | 15 | 21 | 24 |
a Dialysis cut-offs used to separate C32 into different CHOS preparations.
b Standard NMR method used to characterize CHOS fractions. These DPn values are used to name the CHOS fractions.
c Weighted average molar mass are calculated relative to pullulan standards assuming linearity in the entire calibration range (from 6 kDa to 110 kDa) and below.