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. Author manuscript; available in PMC: 2019 Jan 8.
Published in final edited form as: Science. 2013 Aug 9;341(6146):660–664. doi: 10.1126/science.1237150

Fig. 3. Dynamics of translocations in space and time.

Fig. 3.

(A) Mean squared displacement (MSD) plots for LacO and TetO in cells transfected with ISceI or ISceID44A. Values represent means ± SEM of pooled data from three independent experiments (n = 66 to 98). (B) Mean squared change in distance (Δd2) between the LacO and the closest TetO array of cells with intact arrays (ISceID44A, n = 34), DSBs without pairing events (ISceI, n = 33), or persistent colocalization (ISceI, pairing ≥3 hours, n = 43). (C) Translocations occur predominantly from proximally located DSBs. Persistent LacO-TetO pairs (≥3 hours) were identified, and distance histograms between LacO and TetO arrays for the time of pairing (t = 0, light blue bar) and 5 hours before (t = −5 hours, red bars) were generated (n = 40). (D) Average LacO- TetO distances as a function of time before pairing for 1 hour, ≥3 hours, and ≥5 hours (all tracks, n = 2897; 1-hour transient interaction, n = 1437; at least 3 hours, n = 615; 5-hour pairing, n = 157). Values represent means ± SD from two independent experiments.