Fig. 5.

Lamotrigine (LTG) induced a robust increase in B-cell lymphoma-2 (Bcl-2) promoter activity and association with acetylated histone H3 (AH3). (a) Cerebellar granule cells (CGCs), (b) HEK293 cells and SH-SY5Y cells were transfected with a luciferase reporter driven by a 0.3-kb fragment of the human Bcl-2 promoter. Twenty-four hours after transfection, cells were treated for 24 h with 10–200 μM LTG. Bcl-2 promoter activity was assayed using the luciferase reporter system. Data are mean±S.E.M. from three independent experiments. (c) CGCs were treated with 100 μM LTG or vehicle for 24 h and subjected to chromatin immunoprecipitation (ChIP) analysis to detect levels of AH3 associated with the Bcl-2 promoter by semi-quantitative polymerase chain reaction (PCR). (d) Quantified data are expressed as mean±S.E.M. of the relative optical density ratio of Bcl-2 promoter PCR product bands vs. input lane bands and normalized to vehicle-treated controls from three independent experiments. * p<0.05 compared to vehicle-treated control cultures using an unpaired t test.