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. Author manuscript; available in PMC: 2020 Jan 15.
Published in final edited form as: Methods. 2018 Aug 10;153:3–12. doi: 10.1016/j.ymeth.2018.08.002

Fig. 2.

Fig. 2.

Overview of instrumentation and sample cells. (A) Objective-type TIRF microscope. (B) Prism-type TIRF microscope. (C) Pipet tip chamber sample cell. (D) 3D-printed sample cell with cylindrical reservoir and tapered conical base. (E) Sandwich-type sample cell for prism-TIRF measurements. (F)-(G) Scale drawings showing a top view of each sample cell type shown in (C)-(E). The black-shaded region in each panel represents the surface area available for target capture and imaging on the coverslip or slide. Blue-shaded regions in (F) and (G) represent the plastic walls of the sample wells.