Figure 5.

miR-103 Promotes Inflammation by Targeting TRAF3 in Adipocytes

Adipocytes were treated with Ad-GFP, Ad-miR103, or Ad-miR103 + Ad-TRAF3. (A) Relative levels of IL-6, IL-1β, MCP-1, p62, ATG7, and Beclin1 mRNAs. (B) Relative mtROS amounts. (C) Relative concentrations of mtDNA. (D) Intracellular distribution of LC3 and mitochondria. (E) Subcellular distribution of p62, LC3II, and Parkin. (F) Western blot analysis of NLRP3, Caspase 1, IL-1β, p62, and LC3II protein levels with or without CsA treatment. Scale bar, 200 μm. Error bar, SE. Data are represented as the mean ± SEM (*p < 0.05 and **p < 0.01; n ≥ 3).