Figure 2.

CD8⁺ T cm Can Be Infected with VSV and Transfer Virions to Susceptible Cells

CD8⁺ T cm were infected with indicated MOIs of rVSV vectors expressing GFP and cultured until the indicated time points. (A) CD8⁺ T cm were counted. Percentage of viable cells normalized to time point 0 hr are shown. Means ± SD of three independent experiments, performed in triplicates from three different CD8⁺ T cm donors, are shown. Statistical analysis was performed by two-way ANOVA with Bonferroni post-test (*p < 0.05). (B) TCID₅₀ assays were performed from tissue culture supernatants at the indicated time points. The experiment was performed three times in triplicates from different donors, and the means ± SD are shown. Two-way ANOVA with Bonferroni post-test showed no significant difference between the different MOIs at 24 hr after infection. (C) Infected CD8⁺ T cm were co-cultured with BHK-21 cells. The percentage of GFP⁺ BHK-21 cells was used to calculate the viral load on the CD8⁺ T cm surface that could be transferred to other cells. The experiment was performed in triplicates using CD8⁺ T cm derived from three individual donors. Means ± SD are shown. The best-fitting equation is given.