Fig. 3. DHA induces stronger anti-fibrogenic effects than other FAs.

a αSMA, COL1A1 and ADRP expression in primary mHSCs plated for 3 days and treated with DMSO or 100 µM DHA for 48 h. b–d Lx2 cells treated with DMSO or with 50 µM of the indicated PUFAs. αSMA, COL1A1 and ADRP expression at 24 h (b), MTT assay at indicated times (c) and lipid droplet content at 72 h (d). Scale bar, 20 μm. Experiments were performed two (a, b) or three (c) times