Fig. 4.

Distribution of immune cell subsets in tumour draining (TDLN) and non-draining lymph nodes (LN) of 4T1-tumour bearing Balb/c mice. Cell suspensions from lymph nodes obtained from 21- or 28-day-tumour bearing mice were labelled with specific antibodies: a CD4-FITC: T helper lymphocytes marker, b CD8-PE: T cytotoxic lymphocytes marker, c CD3-FITC: T lymphocytes marker, d ratio CD4⁺/CD8⁺, e CD19-PE: B lymphocytes marker, f CD49-PE and CD3-FITC: NK markers, g CD44-FITC: activated T lymphocyte marker; CD4-FITC, CD25-APC, FoxP3-PE: Treg markers. Error bars represent the means ± SEM of three independent experiments (ANOVA and Newman–Keuls Multiple Comparison Test. *P < 0.05 vs. WT; T-test, ^#P < 0.05, ^##P < 0.01, ^###P < 0.001 vs. LN). h TNFα concentration in TDLN-conditioned medium. Error bars represent the means ± SEM of two independent experiments (T-test, *P < 0.05)