Fig. 1. The loss of Ago2 expression correlates with miR-145-5p downregulation in breast cancer FFPE tumor samples.

a Box plot showing the miR-145-5p expression in the various intrinsic subtypes of breast cancer tissues relative to normal tissues by using TGCA data; Clinical information of breast cancer patients and normalized TCGA miRNAseq data were obtained from http://gdac.broadinstitute.org/ (doi. 10.7908/C11G0KM9) and from Genomic Data Commons Data Portal (https://cancergenome.nih.gov/). Deregulation among different subgroups of samples was assessed by one way Anova test and two-sided t-test. b Box plot showing the modulation of miR-145-5p expression in 11 matched triple negative tumor tissues vs normal samples; c, d Immunohistochemistry for Ago2 in slides from 2 representative patients (Pt) with relative scale bars