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. 2019 Jan 8;10:76. doi: 10.1038/s41467-018-08026-8

Fig. 1.

Fig. 1

Thbs3 is expressed in the diseased heart and induces an adaptive ER stress response. a Quantitation of relative protein expression from Western blots for Thbs3 from heart tissue of mice that were sham-operated, subjected to 2 weeks of TAC, that contain the activated calcineurin A transgene (CnA) or that lack the Csrp3 gene. *P < 0.05 vs sham by one-way ANOVA and Turkey multiple comparisons test. Results are from four experiments and error bars represent +/−SEM. b Immunohistochemistry for Thbs3 protein (green), desmin (red) and both merged with DAPI (blue) sham or TAC-operated hearts 12 weeks later. Scale bars are 10 μm. c Representative immunohistochemistry for Thbs3 (red) and PDI (green) from CnA transgenic hearts at 8 weeks of age. Scale bars are 10 μm. d Schematic diagram depicting the inducible bi-transgenic system regulated by tetracycline for inducible overexpression of Thbs3 in the heart. e Representative Western blots for Thbs3, the nuclear form of ATF6α, Armet, BiP, calreticulin (CRT) and Gapdh from hearts of tTA control and Thbs3 DTG mice. f Western blots for Thbs3 from neonatal rat ventricular myocytes (NRVM) infected with recombinant adenovirus expressing Myc-tagged ATF6α and βgal or Thbs3. Input control and immunoprecipitation (IP) of Thbs3 with Myc-tagged ATF6α are shown. g Representative immunohistochemistry for Thbs3 (red), WGA (green) or PDI (green) and DAPI (blue) from hearts of tTA control and Thbs3 DTG mice. Scale bars are 10 μm. h Transmission electron microscopy (EM) of heart sections from tTA control and Thbs3 DTG mice. The white arrows show expanded ER and vesicles only in Thbs3 DTG hearts. Scale bars are 1 μm