Fig. 8.

Thbs3 enhances integrin turnover. a–h Cell surface biotinylated proteins were used for input control experiments or immunoprecipitated with streptavidin (Surface, a–d) and endocytic protein uptake was induced for 30 min followed by immunoprecipitation with streptavidin (Uptake, e–h). Quantitative analysis of protein band intensities of β1D integrin, α5 integrin, calreticulin (CRT), and calnexin (CXN) from NRVMs infected with Adβgal, AdThbs3, AdThbs4, AdThbs3-RGD adenovirus or treated with recombinant Thbs3 protein (western blots used for quantification are presented in the uncropped western blot images attachment). Data are represented as fold expression over Adβgal relative to input controls. *P < 0.05 versus Adβgal. Statistical analysis was performed using one-way ANOVA and Turkey multiple comparisons test. Error bars are +/− standard error of the mean and number of independent experiments are shown in the graphs