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. 2019 Jan 8;10(1):15. doi: 10.1038/s41419-018-1251-0

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a Expression of Th1-type cytokines and transcription factors of dCD4⁺T cells cultured for 48 h in the presence or absence of anti-CTLA-4 antibody (10 μg/ml), anti-Tim-3 antibody (10 μg/ml), or both. b Quantification of flow cytometric analysis of IL-4 and GATA-3 expression by dCD4⁺T cells following treatment with the indicated blocking antibodies. c Expression of Treg-type cytokines by dCD4⁺T cells following treatment with the indicated blocking antibodies. Data represented the mean ± SEM. n = 12. *P < 0.05, **P < 0.01, ***P < 0.001, compared with the control group