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. 2018 Dec 21;63(1):e01618-18. doi: 10.1128/AAC.01618-18

TABLE 1.

Limit of detection of colistin-resistant Enterobacteriaceae on ChromID Colistin R and Superpolymyxin media

Colistin susceptibility and species Strain name Colistin MIC
(mg/liter)		 Colistin resistance
 Lowest LOD (CFU/ml) ina:
 Reference or
source
Plasmid or
chromosome
encodedb 		Mechanism ESwab Amies buffer
 Spiked stools
ChromID
Colistin R		 Superpolymyxin ChromID
Colistin R		 Superpolymyxin
Colistin-resistant Enterobacteriaceae (n = 53)
 Escherichia coli CNR 111 J7 16 Chr PmrB mutations (D14N, S71C, V83A) 1 × 102 1 × 104 1 × 104 1 × 106 21
CNR 20160039 4 Chr Unknown 1 × 102 >1 × 106 1 × 104 >1 × 108 21
CNR 20160235 8 Chr MgrB mutation (V8A) 1 × 105 >1 × 106 1 × 107 >1 × 108 21
CNR 1728 8 Chr PmrB mutation (G160E) 1 × 106 1 × 104 1 × 108 1 × 106 21
41489 4 P mcr-1 1 × 103 1 × 105 1 × 105 1 × 107 21
J53 + mcr-1f 8 P mcr-1 1 × 106 1 × 104 1 × 108 1 × 106 21
CNR20140385 4 P mcr-1 >1 × 106 1 × 104 >1 × 108 1 × 106 21
S08-056 4 P mcr-1 1 × 104 1 × 103 1 × 106 1 × 105 21
CNR 117 G7 4 P mcr-1 >1 × 106 1 × 104 >1 × 108 1 × 106 22
CNR 121 G9 4 P mcr-1 1 × 106 1 × 105 1 × 108 1 × 107 23
R12 F5 4 P mcr-2 1 × 103 >1 × 106 1 × 105 >1 × 108 11
CNR 1745 4 P mcr-1 >1 × 106 1 × 104 >1 × 108 1 × 106 21
CNR 1604 4 P mcr-1 1 × 106 1 × 104 1 × 108 1 × 106 21
CNR 1790 4 P mcr-1 1 × 103 1 × 103 1 × 105 1 × 105 21
CNR 1859 4 P mcr-1 1 × 103 1 × 103 1 × 105 1 × 105 21
CNR 1886 4 P mcr-1 1 × 102 1 × 104 1 × 104 1 × 106 21
TOP10 + mcr-5f 8 P mcr-5 1 × 103 1 × 105 1 × 105 1 × 107 21
4222 4 P mcr-1 1 × 102 1 × 103 1 × 104 1 × 105 21
4070 4 P mcr-1 1 × 104 1 × 103 1 × 106 1 × 105 21
979 4 P mcr-1 1 × 103 1 × 103 1 × 105 1 × 105 21
6383 4 P mcr-1.5 1 × 103 1 × 104 1 × 105 1 × 106 21
1724 4 P mcr-1 1 × 104 1 × 103 1 × 106 1 × 105 21
1670 4 P mcr-1.5 1 × 105 1 × 105 1 × 107 1 × 107 21
36070 8 P mcr-3.2 1 × 105 1 × 105 1 × 107 1 × 107 24
CNR 164 A5 4 P mcr-1 1 × 105c >1 × 106 1 × 107 >1 × 108 This study
 Klebsiella pneumoniae CNR 20140042 16 Chr MgrB N42Y and K43I 1 × 103 1 × 103 1 × 105 1 × 105 This study
CNR 20140661 64 Chr MgrB Q30 stop 1 × 102 1 × 104 1 × 104 1 × 106 This study
CNR 20151119 64 Chr MgrB L4 stop 1 × 102 1 × 104 1 × 104 1 × 106 This study
CNR 20150622 64 Chr MgrB Y41 stop 1 × 102 1 × 103 1 × 104 1 × 105 This study
CNR 20150777 128 Chr MgrB Y41 stop 1 × 103 1 × 104 1 × 105 1 × 106 This study
CNR 20150944 64 Chr MgrB modified sequence starting at aad 42 1 × 102 1 × 103 1 × 104 1 × 105 This study
CNR 20150309 64 Chr MgrB modified sequence starting at aa 37 1 × 102 1 × 104 1 × 104 1 × 106 This study
CNR 20150675 64 Chr mgrB truncated in ORFe by IS10 1 × 102 1 × 104 1 × 104 1 × 106 This study
CNR 20140483 32 Chr mgrB truncated in ORF by IS1F-like 1 × 103 1 × 104 1 × 105 1 × 106 This study
CNR 20140563 64 Chr mgrB truncated in ORF by IS1R 1 × 102 1 × 103 1 × 104 1 × 105 This study
CNR 20150050 32 Chr mgrB truncated in promoter by IS1R 1 × 103 1 × 103 1 × 105 1 × 105 This study
CNR 20140591 64 Chr mgrB truncated in ORF by IS5-like 1 × 102 1 × 104 1 × 104 1 × 106 This study
CNR 20140550 32 Chr mgrB truncated in promoter by IS903D 1 × 103 1 × 104 1 × 105 1 × 106 This study
CNR 20151285 32 Chr mgrB truncated in ORF by IS903-like 1 × 103 1 × 104 1 × 105 1 × 106 This study
S14-002 64 Chr mgrB truncated in promoter by ISKpn14 1 × 102 1 × 104 1 × 104 1 × 106 This study
CNR 20140101 32 Chr ΔmgrB 1 × 103 1 × 104 1 × 105 1 × 106 This study
CNR 2015007 32 Chr ΔmgrB 1 × 103 1 × 104 1 × 105 1 × 106 This study
CNR 20150066 16 Chr ΔmgrB 1 × 103 >1 × 106 1 × 105 >1 × 108 This study
CNR 20151223 32 Chr ΔmgrB 1 × 102 1 × 103 1 × 104 1 × 105 This study
S15 64 Chr mgrB truncated in ORF by ISKpn25 1 × 102 1 × 104 1 × 104 1 × 106 25
CNR 1630 64/32 Chr mgrB truncated in ORF by IS5 1 × 102 1 × 105 1 × 104 1 × 107 This study
CNR 1861 16 Chr PmrB mutation (T157P) 1 × 103 1 × 104 1 × 105 1 × 106 This study
CNR 1601 32 Chr + P mcr-1 + mgrB truncated in ORF by IS5 1 × 102 1 × 104 1 × 104 1 × 106 This study
CNR 1732 4 P mcr-1 1 × 103 1 × 103 1 × 105 1 × 105 This study
CNR 1853 4 P mcr-1 1 × 103 1 × 103 1 × 105 1 × 105 This study
 Salmonella enterica
        Serovar Paratyphi B d-tartrate + S. enterica biotype java 201610686 8 P mcr-1 1 × 103 1 × 105 1 × 105 1 × 107 This study
        Serovar Typhimurium CNR 1776 8 P mcr-1 1 × 103 >1 × 106 1 × 105 >1 × 108 This study
        Serovar Paratyphi B d-tartrate + S. enterica biotype java 13-SA01718 8 P mcr-5 1 × 103 >1 × 106 1 × 105 >1 × 108 8
Colistin-susceptible Enterobacteriaceae (n = 41)
 Escherichia coli TOP10 0.25 >1 × 106 >1 × 106 >1 × 108 >1 × 108 21
1608071881 0.25 >1 × 106 >1 × 106 >1 × 108 >1 × 108 21
1608072264 0.25 >1 × 106 >1 × 106 >1 × 108 >1 × 108 21
1608073733 0.5 >1 × 106 >1 × 106 >1 × 108 >1 × 108 21
1608073228 0.25 >1 × 106 >1 × 106 >1 × 108 >1 × 108 21
1608078635 0.25 >1 × 106 >1 × 106 >1 × 108 >1 × 108 21
1608078858 0.25 >1 × 106 >1 × 106 >1 × 108 >1 × 108 21
1608062671 0.25 >1 × 106 >1 × 106 >1 × 108 >1 × 108 21
1608064819 0.25 >1 × 106 >1 × 106 >1 × 108 >1 × 108 21
2H6 0.25 >1 × 106 >1 × 106 >1 × 108 >1 × 108 21
LAN 10.48 0.25 >1 × 106 >1 × 106 >1 × 108 >1 × 108 21
VER 9.39 0.25 >1 × 106 >1 × 106 >1 × 108 >1 × 108 21
1F1 0.25 >1 × 106 1 × 106 >1 × 108 1 × 108 21
1A6 0.25 >1 × 106 >1 × 106 >1 × 108 >1 × 108 21
1A8 0.25 >1 × 106 >1 × 106 >1 × 108 >1 × 108 21
2A1 0.25 >1 × 106 >1 × 106 >1 × 108 >1 × 108 21
2D9 0.5 >1 × 106 >1 × 106 >1 × 108 >1 × 108 21
2C4 0.25 >1 × 106 >1 × 106 >1 × 108 >1 × 108 21
2D5 0.25 >1 × 106 >1 × 106 >1 × 108 >1 × 108 21
 K. pneumoniae 1609056413 0.5 >1 × 106 >1 × 106 >1 × 108 >1 × 108 This study
1609061149 1 >1 × 106 >1 × 106 >1 × 108 >1 × 108 This study
2 E8 0.5 >1 × 106 >1 × 106 >1 × 108 >1 × 108 This study
2 I4 0.5 >1 × 106 >1 × 106 >1 × 108 >1 × 108 This study
2 F1 0.5 >1 × 106 >1 × 106 >1 × 108 >1 × 108 This study
2 I5 0.5 >1 × 106 >1 × 106 >1 × 108 >1 × 108 This study
3 B4 0.5 >1 × 106 >1 × 106 >1 × 108 >1 × 108 This study
3 B7 0.5 >1 × 106 >1 × 106 >1 × 108 >1 × 108 This study
1 B6 0.5 >1 × 106 >1 × 106 >1 × 108 >1 × 108 This study
CNR 173 F9 0.5 >1 × 106 >1 × 106 >1 × 108 >1 × 108 This study
1 C9 0.5 >1 × 106 >1 × 106 >1 × 108 >1 × 108 This study
1 E3 1 >1 × 106 1 × 104 >1 × 108 1 × 106 This study
2 B1 1 >1 × 106 >1 × 106 >1 × 108 >1 × 108 This study
CNR 173 E3 0.5 >1 × 106 >1 × 106 >1 × 108 >1 × 108 This study
2 C6 0.5 >1 × 106 >1 × 106 >1 × 108 >1 × 108 This study
2 D2 0.5 >1 × 106 >1 × 106 >1 × 108 >1 × 108 This study
 Salmonella enterica
        4,12:i:− 201604739 1 >1 × 106 >1 × 106 >1 × 108 >1 × 108 This study
        Serovar Enteritidis 201608919 1 >1 × 106 >1 × 106 >1 × 108 >1 × 108 This study
        Serovar Typhimurium 201606509 1 >1 × 106 >1 × 106 >1 × 108 >1 × 108 This study
        Serovar Enteritidis 201607559 0.5 >1 × 106 >1 × 106 >1 × 108 >1 × 108 This study
        Serovar Veneziana 201610299 0.5 >1 × 106 >1 × 106 >1 × 108 >1 × 108 This study
 Enterobacter cloacae CNR 131 G4 0.5 P mcr-4.2 >1 × 106 >1 × 106 >1 × 108 >1 × 108 This study
a

Underlined CFU counts are considered negative results. The sensitivity was 86.8% (95% CI = 74.0% to 94.0%) for both media after 48 h of incubation. The specificity was 100% (95% CI = 89.3% to 100%) and 97.5 (95% CI = 85.6% to 99.9%), respectively, for ChromID Colistin R and Superpolymyxin.

b

P, plasmid; Chr, chromosome.

c

After 48 h of incubation (no colony at 24 h).

d

aa, amino acid.

e

ORF, open reading frame.

f

E. coli J53 was a recombinant strain harboring the mcr-1 gene on plasmid pDM1 (41522) (21). E. coli TOP10 was a recombinant strain harboring the mcr-5 gene on plasmid pSE13-SA01718 (21).