TABLE 1.
Liquid MIC values of strains overexpressing wild-type and mutant ScLDM6×His
| Strain | Genotype | MIC80 (nM)a |
||||
|---|---|---|---|---|---|---|
| FLC | VCZ | VT-1161 | ITC | PCZ | ||
| Y2411 | AD2Δ pABC3 | 1,800 ± 50 | 37 ± 0.9 | 29 ± 8 | 91 ± 4 | 160 ± 48 |
| Y2300 | AD2Δ PDR5::ScLDM6×His | 4,200 ± 500 | 93 ± 10 | 75 ± 7 | 180 ± 6 | 423 ± 60 |
| Y2301 | AD2Δ PDR5::ScLDM6×His Y140F ΔERG11 | 8,100 ± 500 | 202 ± 50 | 172 ± 29 | 146 ± 8 | 366 ± 119 |
| Y2513 | AD2Δ PDR5::ScLDM6×His Y140H ΔERG11 | 8,800 ± 1100 | 164 ± 9 | 116 ± 24 | 165 ± 4 | 330 ± 34 |
a
MIC80 determinations were carried out as described in Materials and Methods using SD medium at pH 6.8. Each determination was carried out at least 3 times using samples in triplicate.