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Difference spectrum of Co(II)-substituted wild-type NDM-1 and L3 variants. The difference spectra of the Co(II)-substituted MβLs were obtained by subtraction of the spectrum of the nonmetallated β-lactamase from the one corresponding to the final bi-Co(II) substituted variant. The difference spectrum of the wild-type NDM-1 is shown in blue; that for L3IMP is shown in green, that for L3VIM is shown in red, and that for L3Pro is shown in orange.
