TABLE 1.

Antimicrobial susceptibility profile conferred by β-lactamase-carrying plasmids of E. cloacae WW13 and E. coli WW16 under various genetic backgrounds^d

Antibiotic	Etest MIC (μg/ml) for the following strain host and plasmid β-lactamasesd:
	E. cloacae WW13a ST24 producing OXA-656	E. coli WW16b ST401 producing OXA-655, SHV-12, TEM-1, and OXA-1	trcWW13 E. coli CAG18439(pQGU13, IncFII) producing OXA-656	trcWW16-1 E. coli CAG18439(pQGU16, pEA3-like) producing OXA-655	trcWW16-2 E. coli CAG18439/IncFII(K) producing SHV-12, OXA-1, and TEM-1	E. coli CAG18439 control	trfWW13 E. coli TOP10(pQGU13) producing OXA-656	trfWW16 E. coli TOP10(pQGU16) producing OXA-655	E. coli TOP10 control
β-Lactams
Amoxicillin	>256	>256	>256	>256	>256	6	>256	>256	4
Amoxicillin-clavulanate	64	24	12	8	12	6	12	12	4
Temocillin	16	128	12	32	16	12	16	64	12
Cephalothin	>256	>256	16	12	256	12	12	8	8
Cefuroxime	>256	>256	6	3	8	3	6	3	2
Cefotaxime	16	>32	0.125	0.094	3	0.094	0.125	0.047	0.047
Cefotaxime-clavulanate	>1	0.19	0.032	0.032	0.032	0.032	0.032	0.023	0.023
Ceftazidime	3	>256	0.094	0.094	6	0.094	0.19	0.19	0.125
Cefepime	2	16	0.094	0.032	0.38	0.032	0.094	0.023	0.023
Cefoxitin	>256	>256	4	6	4	3	8	8	8
Aztreonam	3	>256	0.125	0.064	16	0.064	0.38	0.094	0.064
Imipenem	12	1.5	0.25	0.38	0.19	0.19	0.25	0.25	0.25
Meropenem	6	16	0.032	0.125	0.016	0.016	0.023	0.125	0.023
Ertapenem	8	>32	0.016	0.25	0.004	0.003	0.016	0.19	0.002
Doripenem	4	6	0.047	0.125	0.023	0.023	0.032	0.125	0.032
Non β-lactams
Gentamicin	0.5	>256	0.25	0.19	16	0.25	0.38	0.38	0.19
Streptomycin	8	>1,024	6	6	1.5	1.5	>1,024	>1,024	>1,024c
Sulfamethoxazole	>1,024	>1,024	>1,024	>1,024	8	8	>1,024	>1,024	8
Trimethoprim	0.25	>32	0.125	0.125	>32	0.125	0.19	0.19	0.19
Chloramphenicol	1.5	>256	8	8	>256	8	2	2	2
Tetracycline	1.5	2	>256	>256	>256	>256c	1.5	1.5	1.5
Ciprofloxacin	0.75	0.5	ND	ND	ND	ND	ND	ND	ND

^aChromosomal AmpC derepression and OmpC, OmpD, and OmpF loss. See the text.

^bOmpF loss. See the text.

^cResistant host.

^dtrc, transconjugant; trf, transformant; ND, not determined.