TABLE 2.
List of primers used in this study
| Purpose and name | Sequence (3′–5′) |
|---|---|
| CgEPA3 gene cloning | |
| pGREG_CgEPA3_I_FW | TGCTATTAGGTCAACCTTGTAACAGCTGCCATAGCTATTCGAACTATAGCTTAAG |
| pGREG_CgEPA3_I_Rev | CAGTTGCCACGATGACTAGTCAGCTGGAGCTCAGTACATTAATCAATACAGTGCG |
| pGREG_CgEPA3_II_FW | GTCATCGTGGCAACTGTGATTTTCTTTCTAGATTCCTACT |
| pGREG_CgEPA3_II_Rev | CGTGAAAAAAAGACTAAATTCAGCTGGAGCTCAGTACATTAATCAATACAGTGCG |
| pGREG576 GAL-to-PDCI promotor replacement | |
| pGREG_PDCI_ FW | TTAACCCTCACTAAAGGGAACAAAAGCTGGAGCTCAGCATTTTTATACACGTTTTAC |
| pGREG_PDCI_Rev | GAAAAGTTCTTCTCCTTTACTCATACTAGTGCGGCTGTTAATGTTTTTTGGCAATTG |
| CgEPA1, CgEPA3, CgEPA9, CgEPA10, AWP12 and AWP13 gene disruption | |
| ΔCgEPA1_FW | GCACTAGTCGCCGGACAAAATCAAACCAATTAACGTTTCTGATTTAGAGTTCTTACTTCTTTTTCGAAAC |
| ΔCgEPA1_Rev | GGTCGGAGTGCTACATTTTGGTCCTTTATATTATTGAAGGTATCAAATCGTAACATTTTCGACACCACCG |
| ΔCgEPA3_FW | GCACTAGTCGCCGGTAACTAAAAAAAAGAAAACATTGAAAAAGACTAGTTAAATTAAGCTAATACTACCA |
| ΔCgEPA3_Rev | GGTCGGAGTGCTACATTAAGACAAGGGAATTAAAGATAATACTATAACAAAGAGAAATAAGTAACTAATT |
| ΔCgEPA3_SAT1_FW | ATCATTCCTGGTTTGACAATGGAACTTTACTCTTACCCTATTGTCAGTGGTACTCCTCCCATGGACGGTGGTATGTTTTA |
| ΔCgEPA3_SAT1_Rev | GCTTCGAAGCTTTACCCTTGTACTCTGAAAAATTAGGGATATTAAAGACAGAACTAGAGGTTAGGCGTCATCCTGTGCTC |
| ΔCgEPA9_FW | TCACTAAGTGGATGAAATGCAGAAAGAATACAATTTAACCCTCTTCAATGCGATCCGGCGGCCGCTGATCACG |
| ΔCgEPA9_Rev | CAACTGCCAAAGGGTTCACAAGTTGAATACCAGGCCAACCATCATCAGTGGCAGGGCGACATCGTGAGGCTGG |
| ΔCgEPA10_FW | GCACTAGTCGCCGGGCTAATACCTGATCGTAGACTATATTACTTCATAAAACTTTTGTTACCTACCCGTA |
| ΔCgEPA10_Rev | GGGAAAGAAAAATCAACCACATTGGCAAAACGTAGACATCTTGTCAACTGCCAAAGCATCGTGAGGCTGG |
| ΔCgAWP12_FW | TACTTAAAATTTCCTTTCTCAATCAACAAAAATATCCATTAACTGAAAAAGAATACGGCGGCCGCTGATCACG |
| ΔCgAWP12_Rev | TATCAATGTTCATATTGAAAGAAACAGCCTATCTAAAAATCATGGCTGGACGAACGCGACATCGTGAGGCTGG |
| ΔCgAWP13_FW | ATTACGTGACAAAAGACAGATAAAGGAATTCTAAATATCCATCTACAAGACCATTCGGCGGCCGCTGATCACG |
| ΔCgAWP13_Rev | CCTATCTAAAAATCATGGCTGGACGAACGTTTGTATTACTGTACATGTTGGGCATTCGACATCGTGAGGCTGG |
| ΔCgEPA1_conf_FW | TAAATAAGTTTTTATCTCGACC |
| ΔCgEPA1_conf_Rev | GGTTTTCATTGACCGAAG |
| ΔCgEPA3_conf_FW | CGGAACTTCATTGGTATG |
| ΔCgEPA3_conf_Rev | CATACCAATGAAGTTCCG |
| ΔCgEPA3_SAT1_conf_FW | CCCAGAACCCATTTGGTTATCC |
| ΔCgEPA3_SAT1_conf_Rev | GCCCAGATAACAACACAAGTCC |
| ΔCgEPA9_conf_FW | CTTTTGGTATCTGACTCTGTTAT |
| ΔCgEPA9_conf_Rev | GGTCCCAAGAGATTTTACC |
| ΔCgEPA10_conf_FW | TGTCTCAGTCTATGGCTTTC |
| ΔCgEPA10_conf_Rev | CTCAAGTGTCTCCCAACA |
| ΔCgAWP12_conf_FW | GCCTGTGGATATTGCTACT |
| ΔCgAWP12_conf_Rev | GTCACTGATTGAAAGTTCTCG |
| ΔCgAWP13_conf_FW | GTATATTTTCAAGTGCTGCAT |
| ΔCgAWP13_conf_Rev | CTACCGTGGTTTCACTTG |
| RT-PCR experiments | |
| CgEPA1_FW | TTGATTGCTGCAGAAGGGATT |
| CgEPA1_Rev | ATGGCGTAGGCTTGATAATTTCC |
| CgEPA3_FW | GTTCCTGTCACCAGCACCATATACTA |
| CgEPA3_Rev | CCTTGGCAACTAGGTGTTTGG |
| CgCDR1_FW | GCTTGCCCGCACATTGA |
| CgCDR1_Rev | CCTCAGGCAGAGTGTGTTCTTTC |
| CgACT1_FW | AGAGCCGTCTTCCCTTCCAT |
| CgACT1_Rev | TTGACCCATACCGACCATGA |