TABLE 4.
Comparison of 4 plate types in Mueller-Hinton broth versus MRSA ATCC 43300 in the presence and absence of Tween 80 (polysorbate 80) or lysed horse blood (LHB), with added 50% human serum (HS) or 2% lung surfactant (LS)
| Plate | Medium | Additive | MIC (µg/ml) by antibiotic |
||||||
|---|---|---|---|---|---|---|---|---|---|
| Vancomycin | Ciprofloxacin | Telavancin | Dalbavancin | MCC223 | MCC310 | MCC520 | |||
| Corning NBS | MH | None | 0.5 | 0.125 to 0.25 | 0.03 to 0.06 | 0.03 to 0.06 | ≤0.003 | ≤0.003 | 0.007 |
| 50% HS | 0.25 to 0.5 | 1 | 0.25 | >8 | 0.125 | 0.125 | 0.03 | ||
| 2% LS | 0.5 | 0.125 to 1 | 0.125 | 0.25 to 0.5 | 0.03 to 0.06 | 0.015 to 0.03 | 0.015 to 0.03 | ||
| MH + T80 | None | 1 | 0.5 | 0.125 | 0.125 to 0.5 | 0.003 to 0.007 | 0.007 | 0.015 | |
| 50% HS | 1 | 0.125 to 0.25 | 0.25 | >8 | 0.125 | 0.06 | 0.03 to 0.06 | ||
| 2% LS | 0.25 to 0.5 | 0.25 to 0.5 | 0.06 to 0.125 | 0.25 to 0.5 | 0.125 | 0.06 to 0.125 | 0.03 | ||
| MH + 2% LHB | None | 1 | 0.06 to 0.5 | 0.06 to 0.125 | 0.06 to 0.25 | 0.06 to 0.125 | 0.06 | 0.03 | |
| 50% HS | 0.5 to 1 | 0.25 to 0.5 | 0.25 | 8 | 0.125 | 0.06 to 0.125 | 0.06 to 0.125 | ||
| 2% LS | 0.5 to 1 | 0.5 to 1 | 0.125 | 0.25 | 0.25 | 0.015 to 0.06 | 0.015 | ||
| Corning PS | MH | None | 0.5 | 0.25 | 0.06 | 0.5 to 1 | 0.5 to 1 | 4 | 0.125 to 0.25 |
| 50% HS | 0.25 to 0.5 | 0.25 to 1 | 0.125 to 0.25 | >8 | 0.06 to 0.25 | 0.06 to 0.125 | 0.06 to 0.25 | ||
| 2% LS | 0.5 to 1 | 0.5 to 2 | 0.03 to 0.125 | 1 to 2 | 1 to 2 | 1 to 2 | 0.03 to 0.06 | ||
| MH + T80 | None | 1 to 2 | 0.25 to 0.5 | 0.03 to 0.06 | 0.03 to 0.125 | 0.003 to 0.007 | ≤0.003 | 0.007 | |
| 50% HS | 0.5 | 0.125 | 0.25 | >8 | 0.125 to 0.25 | 0.06 to 0.125 | 0.03 to 0.125 | ||
| 2% LS | 0.5 to 2 | 0.25 to 0.5 | 0.06 to 0.25 | 0.25 to 0.5 | 0.125 to 0.25 | 0.06 to 0.25 | 0.03 to 0.06 | ||
| MH + 2% LHB | None | 1 | 0.25 to 1 | 0.06 | 0.25 to 0.5 | 0.25 to 0.5 | 0.25 to 0.5 | ≤0.003 | |
| 50% HS | 0.5 to 1 | 0.03 to 0.5 | 0.125 to 0.25 | >8 | 0.25 to 0.5 | 0.125 to 0.25 | 0.03 to 0.06 | ||
| 2% LS | 0.5 to 1 | 0.5 | 0.125 | 0.125 to 0.25 | 0.25 to 0.5 | 0.25 to 1 | 0.015 to 0.03 | ||
| Corning TC | MH | None | 0.5 | 0.06 to 0.125 | 1 | 4 | 1 to 2 | 2 to 4 | 2 to 4 |
| 50% HS | 0.25 to 0.5 | 0.5 to 1 | 0.5 to 1 | >8 | 4 to >8 | 4 to >8 | 1 to >8 | ||
| 2% LS | 0.25 to 1 | 0.125 to 0.5 | 0.25 to 0.5 | 1 | 4 to 8 | 2 to 4 | 1 | ||
| MH + T80 | None | 1 | 0.125 to 0.5 | 0.125 to 0.25 | 0.125 to 0.25 | 1 to 2 | 2 to 4 | 4 | |
| 50% HS | 0.5 to 1 | 0.25 to 1 | 0.25 to 1 | >8 | 2 to 8 | 4 to 8 | 2 | ||
| 2% LS | 0.5 | 0.5 to 1 | 0.125 to 0.25 | 0.25 to 0.5 | 4 | 4 to 8 | 2 | ||
| MH + 2% LHB | None | 0.5 | 0.125 | 0.125 | 0.06 to 0.5 | 2 to 4 | 2 to 8 | 1 to 4 | |
| 50% HS | 0.5 | 0.25 to 1 | 2 to 8 | 8 | 2 to 8 | 0.5 to 4 | 2 to 4 | ||
| 2% LS | 0.5 to 1 | 0.5 | 0.25 to 1 | 0.25 to 0.5 | 4 to >8 | 4 | 2 to 4 | ||
| Trek PS | MH | None | 0.5 to 1 | 0.5 to 1 | 0.03 to 0.06 | 0.25 to 0.5 | 0.5 to 1 | 4 | 0.125 |
| 50% HS | 0.25 | 0.25 to 2 | 0.125 | >8 | 0.06 to 0.25 | 0.06 to 0.125 | 0.003 to 0.015 | ||
| 2% LS | 0.03 | 0.125 to 0.25 | 0.03 to 0.06 | 1 | 0.25 to 0.5 | 0.25 to 1 | 0.25 to 1 | ||
| MH + T80 | None | 1 | 0.25 to 0.5 | 0.03 to 0.06 | 0.06 | ≤0.003 | ≤0.003 | 0.007 | |
| 50% HS | 0.25 to 0.5 | 0.25 to 0.5 | 0.125 to 0.25 | 8 to >8 | 0.125 | 0.06 | 0.03 to 0.06 | ||
| 2% LS | 0.06 to 1 | 0.25 to 0.5 | 0.06 | 0.25 | 0.25 | 0.25 | 0.015 | ||
| MH + 2% LHB | None | 0.25 | 0.06 to 0.125 | 0.015 | 0.125 | 0.125 | 0.125 | 0.003 to 0.015 | |
| 50% HS | 0.5 | 0.25 to 0.5 | 0.06 to 0.125 | 8 to >8 | 0.125 to 0.25 | 0.06 to 0.5 | 0.06 to 0.25 | ||
| 2% LS | 0.25 to 0.5 | 0.125 | 0.06 to 0.25 | 0.125 | 0.25 | 0.125 | ≤0.003 | ||