TABLE 4.
Synergy/antagonism analysis for NVR 3-778 in combination with nucleoside analogs using MacSynergy and CalcuSyn
| Analog used in combination with NVR 3-778 |
Synergy (μM2%) |
Antagonism (μM2%) |
MacSynergy-predicted effecta |
CI valueb |
Overall CIc | CalcuSynb-predicted effectd | ||
|---|---|---|---|---|---|---|---|---|
| ED50 | ED75 | ED90 | ||||||
| LMV | 5.1 | −14.3 | Additive | 1.0 | 0.8 | 0.8 | 0.9 ± 0.1 | Additive |
| TFV | 18.5 | −7.3 | Additive | 0.8 | 0.8 | 0.8 | 0.8 ± 0.06 | Slight to moderate synergy |
| ETV | 1.0 | −16.5 | Additive | 1.0 | 0.5 | 0.5 | 0.7 ± 0.4 | Slight to moderate synergy |
Synergy/antagonism volumes at 95% confidence of <25 µM2% were defined as insignificant, those between 25 and 50 µM2% were defined as minor, those between 50 and 100 µM2% were defined as moderate, and those of >100 µM2% were defined as strong.
CI, confidence interval. The confidence intervals at ED50, ED75, and ED90 represents mean values determined from at least 5 different combination ratios.
Mean and standard deviation of all confidence interval (CI) values.
The combination effect prediction based on overall confidence interval values is as follows: <0.7 is synergy, 0.7 to 0.9 is slight to moderate synergy, 0.9 to 1.1 is additive, 1.1 to 1.5 is slight to moderate antagonism, and >1.5 is antagonism.