FIG 5.

The Candida albicans erg3Δ/Δ mutant sustains membrane damage following fluconazole treatment. The Candida albicans WT strain (GP1), the erg3Δ/Δ mutant, erg3Δ/Δ + ERG3, a group of trailing isolates (SC5314, JS7, JS17, JS22, and JS23), and a set of azole-resistant isolates (JS28, JS29, JS40, and TW17) were transformed with plasmid pDUPNluc. The resulting strains were then grown in 96-well plates in YNB medium with 5 µg/ml fluconazole or 0.5% DMSO (no-drug control). After 24 h of incubation at 35°C, culture supernatant was removed from each well and Nluc activity was measured using a luminescent substrate. Nluc activity was normalized with respect to growth (OD₆₀₀), and results are expressed relative to the no-drug controls for each strain. The means ± standard deviations for three independent transformants of each strain are shown. The means for the experimental groups were compared using one-way analysis of variance and Tukey's multiple-comparison test. The P values for each strain, compared to the erg3Δ/Δ mutant, are indicated.