FIG 2.
Protein-protein interactions using coexpression and copurification. The proteins indicated below each panel (A to K) were coexpressed in E. coli and copurified on a nickel column using one (His)-tagged protein as a bait. The eluted proteins were then labeled with fluorescent ampicillin, when PBPs were present, and analyzed by SDS-PAGE followed by fluorescence (FL) imaging and protein staining with Coomassie blue (CB). M, protein standard; EL, elution fractions from Ni affinity column; FL, fluorescently labeled PBPs; Ex, protein extracts; FT, indicates the flowthrough fractions. The bands of the proteins are indicated by arrowheads. FtsW* is a degradation product of FtsW. α-HA indicates immunoblotting (IM) analysis using antibodies against the HA epitope of FtsWHA. Numbers at left of panels are molecular masses in kilodaltons.