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. 2019 Jan 8;10(1):e01912-18. doi: 10.1128/mBio.01912-18

FIG 5.

FIG 5

Effect of FtsBLQ subunits and mutants and FtsN mutants on the activity of PBP1b. The GTase activity of PBP1b was measured by continuous fluorescence assay (the concentration of PBP1b and other proteins and detergent concentration were optimized for each experiment). (A and B) Comparison of the effects of FtsBLQ with the subunit FtsQ or FtsBL, respectively, on the activity of PBP1b. (C) Comparison of the effect of FtsBLQ containing the mutation FtsLD93A and control complex on the activity of PBP1b. (D) Comparison of the effects of the FtsNW83L and FtsNY85W mutants and wild-type FtsN on the activity of PBP1b. (E) Comparison of the effects of FtsN and FtsNW83L mutant on PBP1b inhibited by FtsBLQ. (F) Effect of FtsBLQ containing the mutation FtsBD59H on the activity of PBP1b.